Coherent Raman Microscopy

30.05.2017, 14:00

Prof. Albert Stolow Canada Research Chair in Molecular Photonics, University of Ottawa and Molecular Photonics Group, National Research Council Canada

Based on third order nonlinear optics, Coherent Raman Microscopy (CRM), which includes Coherent Anti-Stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS), is becoming increasingly popular as ultrashort pulse broadband light sources become increasingly available. A key driver in the ability to perform rapid, 3D label-free yet chemical-specific micro-imaging.

We discuss the basic physics of coherent Raman vibrational spectroscopy and the nonlinear image formation process, and illustrate some of the applications of CRM to biomedical, materials and geological research.

References:

  • M. Andreana,. M-A. Houle, D.J. Moffatt, A. Ridsdale, E. Buettner, F. Légaré, A. Stolow. "Amplitude and polarization modulated hyperspectral Stimulated Raman Scattering Microscopy" Optics Express 23, 28119 (2015)
  • A.F. Pegoraro, A.D. Slepkov, A. Ridsdale, D.J. Moffatt, A. Stolow. "Hyperspectral multimodal CARS microscopy in the fingerprint region" Journal of Biophotonics 7, 49 (2014)
  • A.M. Barlow, K. Popov, M. Andreana, D.J. Moffatt, A. Ridsdale, A.D. Slepkov, J.L. Harden, L. Ramunno, A. Stolow. "Spatial-Spectral Coupling in Coherent Anti-Stokes Raman Scattering Microscopy". Optics Express 21, 15298 (2013)
  • K.I. Popov, A.F. Pegoraro, A. Stolow, L. Ramunno. "Image formation in CARS and SRS: effect of an inhomogeneous nonresonant background medium" Optics Letters 37, 473 (2012)
  • K.I. Popov, A.F. Pegoraro, A. Stolow, L. Ramunno. "Image Formation in CARS Microscopy: Effect of the Gouy Phase Shift" Optics Express 19, 5902 (2011)
  • A.D. Slepkov, A. Ridsdale, H-N Wan, M-H Wang, A.F. Pegoraro, D.J. Moffatt, J.P. Pezacki, F-J Kao, A. Stolow. "Forward-collected simultaneous fluorescence lifetime imaging and coherent anti-Stokes Raman scattering microscopy" Journal of Biomedical Optics 16, 021103 (2011)
  • A. Pegoraro, A. Slepkov, A. Ridsdale, J.P. Pezacki & A. Stolow. "Single laser source for multimodal CARS microscopy" Applied Optics 49, F10 (2010)
  • A.F. Pegoraro, A. Ridsdale, D.J. Moffatt, J.P. Pezacki, A. Stolow, B.K. Thomas, L. Fu, L. Dong, M.E. Fermann. "All-fiber CARS microscopy of live cells" Optics Express 17, 20700 (2009)
  • A.F. Pegoraro, A. Ridsdale, D.J. Moffatt, Y. Jia, J.P. Pezacki & A. Stolow. "Optimally chirped multimodal CARS microscopy based on a single Ti:sapphire oscillator" Optics Express 17, 2984 (2009)