Alumni

Conferences• 2012 ASCB, Boston (Poster:’ Mechano-sensing of cells on visoelastic biomembrane-mimicking substrates’)
• 2012 4th, International Symposium-Interface Biology of Implants , Warnemünde (Talk:'Mechano-sensing of cells on viscous biomembrane-mimicking substrates')
• 2012 DPG Tagung, Berlin (Talk: Viscosity-Sensing and Mechano-Transduction of Cells on Adhesive Lipid Bilayers')
• 2011 4th European Cell Mechanics Meeting, Amsterdam (Talk: 'Viscosity-Sensing and Mechano-Transduction in Mouse Embryonic Fibroblasts on Adhesive Lipid Bilayers')
• 2010 DPG Tagung, Regensburg (Talk: ' Probing mechanical characteristics of differentiating pluripotent mouse stem cells')
• 2009 DPG Tagung, Dresden
Awards• Best Junior Speakers Award, Cell Mech 2011
Fellowships• Deutsche Physikalische Gesellschaft
Collaborations• Prof. Christoph Naumann, Indiana University-Purdue University, Department of Chemistry and Chemical Biology, Indianapolis
• Pamela Strissel, University of Erlangen_Nuremberg, Universitäts Klinikum- Frauenklinik, Erlangen
Current research / employment Adherent cells sense and actively probe the mechanical properties of their environment which impacts cellular fate and function. Previous studies of cellular sensing were mostly conducted on polymeric cell substrates of adjustable elasticity with immobilized cell-substrate linkers, that do not resemble the dynamic and viscoelastic nature of tissues. An open question remained if these substrates along with changes in elasticity also change the cell linker tethering. We used an alternative way of cell substrate design based on a polymer-tethered multi-lipid bilayer system with distinct material properties. The polymer-tethered lipids connecting the bilayer stacks do not hinder the lateral mobility of individual cell linker molecules in the fluid lipid bilayer matrix, but obstruct or even prevent the free lateral diffusion of clusters of cell linkers. Our biomembrane-mimicking substrates exhibit a viscous response with respect to individual linkers and an elasto-plastic response in the presence of linker clusters and thus allow the cells to rearrange and recruit ligands. Importantly the mechanical properties of these multi-bilayer substrate can be tuned by the number of stacked bilayers. To characterize the new substrates we used fluorescence recovery after photobleaching and a magnetic tweezer setup. Light microscopy methods in combination with image analyzing methods (e.g. cell motility tracking) allow us to investigate changes in cell spreading area and motility as a response to the new substrates. With special assay like 2D traction microscopy we are able to learn about the forces cells generate on the different substrates. To study structural changes within the cell fluorescent protein labeling in combination with total internal reflection (TIRF) and confocal imaging are applied. The new substrates are especially great to study cell focal adhesions since the low height of the substrate (under 50nm) allows for TIRF microscopy.
Publications L. A. Lautscham, C. Y. Lin, V. Auernheimer, C. A. Naumann, W. H. Goldmann, B. Fabry, Biomembrane-mimicking lipid bilayer system as a mechanically tunable cell substrate, Biomaterials, , , (2014)

C. Battle, L. Lautscham, and C. F. Schmidt, Differential interference contrast microscopy using light-emitting diode illumination in conjunction with dual optical traps, Rev. Sci. Instrum., 84, , (2013)

J. Ivanovska, A. Tregubova, V. Mahadevan, S. Chakilam, M. Gandesiri, N. Benderska, B. Ettle, A. Hartmann, S. Söder, E. Ziesché, T. Fischer, L. Lautscham, B. Fabry, G. Segerer, A. Gohla, R. Schneider-Stock, Identification of DAPK as a scaffold protein for the LIMK/cofilin complex in TNF-induced apoptosis, The International Journal of Biochemistry & Cell Biology , , , (2013)

N. Bonakdar, J. Luczak, L. Lautscham, M. Czonstke, T.M. Koch, A. Mainka, T. Jungbauer, W. H. Goldmann, R. Schröder, B. Fabry, Biomechanical characterization of a desminopathy in primary human myoblasts, Biochem Biophys Res Commun., , , (2012)

Y.-H. Lin, D. E. Minner, L. Lautscham, A. Schoenborn, W. Goldmann, B. Fabry, C. A. Naumann, Cellular Mechano-Stimulation by Adjusting the Viscous Drag of Cell-Substrate Linkers in Biomembrane-Mimicking Cell Substrates, Biophysical Journal, 102, , (2012)

J. Breyer, J. Samarin, M. Rehm, L. Lautscham, B. Fabry, M. Goppelt-Struebe, Inhibition of Rho kinases increases directional motility of microvascular endothelial cells, Biochemical Pharmacology, 83, 616-626, (2011)